TY - JOUR
T1 - Amperometric and voltammetric determination of oxytetracycline in trout salmonid muscleusing multi-wall carbon nanotube, ionic liquid and gold nanoparticle film electrodes
AU - Nagles, Edgar
AU - Alvarez, Paola
AU - Arancibia, Verónica
AU - Baez, Mauricio
AU - Garreton, Virginia
AU - Ehrenfeld, Nicole
PY - 2012/12/20
Y1 - 2012/12/20
N2 - The use of a multi-wall carbon nanotube, ionic liquid and gold nanoparticle film glassy carbon electrode to determine oxytetracycline (OTC), one of the mostly used antibiotics in salmon industry, by amperometry and adsorptive stripping voltammetry (AdSV) is reported. Electrodes were coated with [BMIM]3F5EPF3, [EMIM]F3MSO3, [BMIM]PF6and [BMIM]BF4, achieving the highest sensitivity with [BMIM]BF4. Using AdSV, variables like pH, scan rate (ν), accumulation potential, and time (Eacc, tacc) were optimized. The best experimental conditions were: pH = 7.0; ν = 0.60 V s-1; Eacc = 0.40 V, and tacc = 70 s. The relative standard deviation obtained was 1.5% (n=5) for a solution containing 5.0x10-6 mol L-1 of OTC. Using AdSV and amperometrictechniques, the linear calibration curves ranged up to 8.0x10-6 mol L-1 and the detection limits (3σ) were 1.5x10-7 and 2.0x10-8 mol L-1, respectively. The methods were validated using fish tissue spiked with OTC. Because the sensitivity of the amperometric technique is higher than AdSV, the analysis of fish tissue samples was made using the former technique, getting aLoD of 5.3x10-10mol L-1. © 2012 by ESG.
AB - The use of a multi-wall carbon nanotube, ionic liquid and gold nanoparticle film glassy carbon electrode to determine oxytetracycline (OTC), one of the mostly used antibiotics in salmon industry, by amperometry and adsorptive stripping voltammetry (AdSV) is reported. Electrodes were coated with [BMIM]3F5EPF3, [EMIM]F3MSO3, [BMIM]PF6and [BMIM]BF4, achieving the highest sensitivity with [BMIM]BF4. Using AdSV, variables like pH, scan rate (ν), accumulation potential, and time (Eacc, tacc) were optimized. The best experimental conditions were: pH = 7.0; ν = 0.60 V s-1; Eacc = 0.40 V, and tacc = 70 s. The relative standard deviation obtained was 1.5% (n=5) for a solution containing 5.0x10-6 mol L-1 of OTC. Using AdSV and amperometrictechniques, the linear calibration curves ranged up to 8.0x10-6 mol L-1 and the detection limits (3σ) were 1.5x10-7 and 2.0x10-8 mol L-1, respectively. The methods were validated using fish tissue spiked with OTC. Because the sensitivity of the amperometric technique is higher than AdSV, the analysis of fish tissue samples was made using the former technique, getting aLoD of 5.3x10-10mol L-1. © 2012 by ESG.
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M3 - Article
SP - 11745
EP - 11757
JO - International Journal of Electrochemical Science
JF - International Journal of Electrochemical Science
SN - 1452-3981
ER -